B-Glucans in the Caryopsis of Sorghum Bicolor (L.) Moench
نویسندگان
چکیده
Fluorescence microscopy was used to determine the l ocation of Bglucans in sorghum. Sections from three genetically different sorghum cultivars were st ained with Calcofluor or Congo Red , fluorochromes which have been reported to react with 13-glucans. Autofluorescence, indicative of ferulic acid in other cereals, was observed in untreated sections. When stained sections were treated with endo -B-gl ucanase , fluorescence was reduced or entirely eliminated in pericarp, aleurone and endosperm cell walls. 13-Glucans were i so lated from the endosperm of three sorghum cultivars. When reacted with the two dyes, Cal cofl uor or Congo Red , precipitates formed immediately, a reaction which is similar to that produced by mixed linkage B-glucans from other ce r e a 1 s . Initial paper received August 29, 1983. Final manuscr ip t received November 10, 1983. Direct inquiries to C. F. Earp. Telephone number: 4D9-845-2925.
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تاریخ انتشار 2015